pYEULCGT vector (V001896)

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LOCUS       V001896                 7805 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001896
VERSION     V001896
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Ward AC, Castelli LA, Macreadie IG, Azad AA.
  TITLE     Vectors for Cu(2+)-inducible production of glutathione
            S-transferase-fusion proteins for single-step purification from
            yeast
  JOURNAL   Yeast 10 (4), 441-449 (1994)
   PUBMED   7941730
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   Ward AC.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-1993) Alister C. Ward, Medicine, University of
            Melbourne, Royal Melbourne Hospital, Parkville, Vic 3050, Australia
REFERENCE   3  (bases 1 to 7805)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7805)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
            date: "1994"; volume: "10"; issue: "4"; pages: "441-449"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-JUN-1993) Alister C. Ward, Medicine, University of Melbourne,
            Royal Melbourne Hospital, Parkville, Vic 3050, Australia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(4..1350)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(1464..2264)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(2265..2480)
                     /label="URA3 promoter"
     promoter        2596..2700
                     /label="AmpR promoter"
     CDS             2701..3558
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3732..4320
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4608..4629
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4644..4674
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4682..4698
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4706..4722
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5024..5671
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             5678..5695
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             5700..5873
                     /gene="CUP1-1"
                     /label="Copper metallothionein 1-1"
                     /note="Copper metallothionein 1-1 from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P0CX80"
     CDS             complement(6344..7435)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"