pYEG vector (V001899)

LOCUS       V001899                 8670 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001899
VERSION     V001899
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Kobayashi H.
  TITLE     Inducible suppression of global translation by overuse of rare
            codons
  JOURNAL   Appl. Environ. Microbiol. 81 (7), 2544-2553 (2015)
   PUBMED   25636849
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Kobayashi H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2015) Contact:Hideki Kobayashi Agency for
            Marine-Earth Science and Technology, Institute of Biogeosciences;
            2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan URL
            :http://www.jamstec.go.jp/j/
REFERENCE   3  (bases 1 to 8670)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2015"; volume: "81"; issue: "7"; pages:
            "2544-2553"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-JAN-2015) Contact:Hideki Kobayashi Agency for Marine-Earth
            Science and Technology, Institute of Biogeosciences; 2-15
            Natsushima, Yokosuka, Kanagawa 237-0061, Japan URL
            :http://www.jamstec.go.jp/j/"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        152..256
                     /label="AmpR promoter"
     CDS             257..1114
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1288..1876
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2062..2202)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     misc_feature    complement(2903..3195)
                     /label="CEN4"
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      complement(3196..3649)
                     /direction=LEFT
                     /label="ARS1"
                     /note="S. cerevisiae autonomously replicating sequence
                     ARS1"
     promoter        3651..3866
                     /label="URA3 promoter"
     CDS             3867..4667
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        4750..5191
                     /label="GAL1 promoter"
                     /note="inducible promoter, regulated by Gal4"
     promoter        5223..5241
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5255..5968
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     terminator      6068..6315
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             6694..7896
                     /label="AurR"
                     /note="aureobasidin A-resistant mutant of S. cerevisiae
                     inositol phosphorylceramide synthase (Aeed et al., 2009)"
     CDS             complement(8214..8606)
                     /gene="MRP17"
                     /label="Small ribosomal subunit protein bS6m"
                     /note="Small ribosomal subunit protein bS6m from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P28778"