pYD14 vector (V001906)

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LOCUS       V001906                 8248 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001906
VERSION     V001906
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Deng Y, Olson DG, Zhou J, Herring CD, Joe Shaw A, Lynd LR.
  TITLE     Redirecting carbon flux through exogenous pyruvate kinase to achieve
            high ethanol yields in Clostridium thermocellum
  JOURNAL   Metab. Eng. 15, 151-158 (2013)
   PUBMED   23202749
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   Zhou J, Olson DG, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2013) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, U.S
REFERENCE   3  (bases 1 to 8248)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8248)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            15, 151-158 (2013)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-NOV-2013) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, U.S"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..455)
                     /direction=LEFT
                     /label="Clostridium thermocellum"
                     /note="Clostridium thermocellum"
     CDS             complement(561..1418)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1419..1523)
                     /label="AmpR promoter"
     misc_feature    1613..2012
                     /label="similar to Clo1313_1879"
                     /note="similar to Clo1313_1879"
     regulatory      2016..2590
                     /gene="GAPDH"
                     /regulatory_class="promoter"
     gene            2016..2590
                     /gene="GAPDH"
                     /label="GAPDH"
     CDS             2591..3238
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             3259..3804
                     /codon_start=1
                     /locus_tag="or0940"
                     /product="hypoxanthine phosphoribosyl transferase"
                     /EC_number="2.4.2.8"
                     /label="or0940"
                     /protein_id="AHH35087.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            3259..3804
                     /locus_tag="or0940"
                     /label="or0940"
     regulatory      3819..3858
                     /label="hairpin"
                     /note="hairpin"
                     /regulatory_class="terminator"
     misc_feature    4391..4813
                     /label="similar to Clo1313_1879"
                     /note="similar to Clo1313_1879"
     terminator      4846..5034
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(5294..5882)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5969..6547)
                     /codon_start=1
                     /gene="tdk"
                     /locus_tag="or0326"
                     /product="thymidine kinase"
                     /EC_number="2.7.1.21"
                     /label="tdk"
                     /protein_id="AHH35090.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(5969..6547)
                     /gene="tdk"
                     /locus_tag="or0326"
                     /label="tdk"
     regulatory      complement(6548..7168)
                     /gene="cbp"
                     /regulatory_class="promoter"
     gene            complement(6548..7168)
                     /gene="cbp"
                     /label="cbp"
     CDS             complement(7247..8248)
                     /label="repB"
                     /note="RepB replication protein"