Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V001976 | pYBA-1132 | In stock, 1 week for quality controls |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pYBA-1132
- Length:
- 6566 bp
- Type:
- Binary vector
- Replication origin:
- pBBR1 oriV
- Host:
- Plants
- Source/Author:
- Yao L, Yan X, Wang H, Cao M, Robaglia C.
- Promoter:
- CaMV 35S
pYBA-1132 vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pYBA-1132 vector Sequence
LOCUS 40924_47598 6566 bp DNA circular SYN 18-DEC-2018
DEFINITION Binary vector pYBA-1132, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6566)
AUTHORS Yao L, Yan X, Wang H, Cao M, Robaglia C.
TITLE Direct Submission
JOURNAL Submitted (19-NOV-2013) Beijing Agro-Biotechnology Research Center,
Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang
Huayuan Zhonglu, Beijing, Beijing 100097, P.R. China
REFERENCE 2 (bases 1 to 6566)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 6566)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(19-NOV-2013) Beijing Agro-Biotechnology Research Center, Beijing
Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan
Zhonglu, Beijing, Beijing 100097, P.R. China"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6566
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(1..589)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
rep_origin 741..1510
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
CDS 1511..2170
/label=pBBR1 Rep
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
misc_feature 2368..2391
/label=RB 'overdrive' sequence
/note="RB 'overdrive' sequence"
misc_feature complement(2392..2416)
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
misc_feature 2428..2628
/note="CaMV poly-A signal; derived from pRT103"
CDS complement(2645..3361)
/label=EGFP
/note="enhanced GFP"
misc_feature complement(3362..3469)
/label=MCS
/note="pBluescript multiple cloning site"
promoter complement(3474..3819)
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
terminator complement(3921..4173)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(4177..4968)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
promoter complement(4977..5160)
/label=NOS promoter
/note="nopaline synthase promoter"
misc_feature complement(5295..5319)
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS complement(5509..6300)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"