pYB100 vector (V001996)

LOCUS       V001996                 5660 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001996
VERSION     V001996
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Boum Y, Myllykallio H.
  TITLE     Synthetic gene construct for expressing human thymidylate synthase
            in Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Boum Y, Myllykallio H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2008) Institute of Genetics and Microbiology, U.
            Paris Sud, Bat. 409, Orsay 91405, France
REFERENCE   3  (bases 1 to 5660)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5660)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-FEB-2008) Institute of Genetics and Microbiology, U. Paris Sud,
            Bat. 409, Orsay 91405, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             127..144
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             151..1089
                     /gene="TYMS"
                     /label="Thymidylate synthase"
                     /note="Thymidylate synthase from Homo sapiens. Accession#:
                     P04818"
     terminator      1117..1211
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             1255..1911
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     terminator      1979..2065
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(2124..2145)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2161..3240)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(3241..3318)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    3510..3650
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3836..4424)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4598..5455)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5456..5560)
                     /label="AmpR promoter"