pYAT7 vector (V001997)

LOCUS       V001997                 8694 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001997
VERSION     V001997
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Khatib A, Arhab Y, Bentebibel A, Abousalham A, Noiriel A.
  TITLE     Reassessing the Potential Activities of Plant CGI-58 Protein
  JOURNAL   PLoS ONE 11 (1), E0145806 (2016)
   PUBMED   26745266
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Khatib A, Arhab Y, Bentebibel A, Abousalham A, Noiriel A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2015) Organization and Dynamics of Biological
            Membranes, Insitut de Chimie et de Biochimie Moleculaires et
            Supramoleculaires, 43 bd du 11 Novembre 1918, Villeurbanne 69622,
            France
REFERENCE   3  (bases 1 to 8694)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8694)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2016"; volume: "11"; issue: "1"; pages: "E0145806"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-OCT-2015) Organization and Dynamics of Biological Membranes,
            Insitut de Chimie et de Biochimie Moleculaires et Supramoleculaires,
            43 bd du 11 Novembre 1918, Villeurbanne 69622, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(220..322)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        1505..1533
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        1884..1961
                     /label="lacI promoter"
     CDS             1962..3041
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    3057..3078
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3093..3123
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    3131..3147
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3167..3340
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
     CDS             3647..6295
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     CDS             complement(join(8257..8694,1..219))
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"