pYAA-ZP-MCS vector (V002003)

LOCUS       40924_47458        6236 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYAA-ZP-MCS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Zaltsman AA.
  TITLE     pYAA-ZP-MCS: plasmid for expression, localization, and tagging in 
            yeast with an adenine biosynthesis reporter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Zaltsman AA.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2008) Biochemistry and Cell Biology, State 
            University of New York, Stony Brook, NY 11794, USA
REFERENCE   3  (bases 1 to 6236)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6236)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2008) Biochemistry and Cell Biology, State University of New
            York, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        199..574
                     /label=ADE2 promoter
     CDS             575..2287
                     /label=ADE2
                     /note="phosphoribosylaminoimidazole carboxylase, required
                     for adenine biosynthesis"
     rep_origin      complement(2495..2950)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3095..3111
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3121..3139
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3241..3396
                     /note="multiple cloning site containing ISCEI, PIPSPI,
                     ICEUI, and IPPOI unique sites"
     primer_bind     complement(3410..3426)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3463..3481)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3502..3518)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3526..3542)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3550..3580)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3595..3616)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3904..4492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4666..5523)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5524..5628)
                     /label=AmpR promoter
     misc_feature    5664..6167
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"