pY1-gLUC59(MCS) vector (V002004)

LOCUS       40924_47443        5106 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pY1-gLUC59(MCS), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Bonaldi K, Li Z, Kang SE, Breton G, Pruneda-Paz JL.
  TITLE     Novel surface luciferase reporter for high-throughput quantitative 
            test one-hybrid screens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Pruneda-Paz JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2017) Biological Sciences, University of 
            California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 5106)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5106)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-FEB-2017) Biological Sciences, University of California San 
            Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..45
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      46..184
                     /note="from pLacZi; CYC1"
                     /regulatory_class="promoter"
     misc_feature    185..244
                     /gene="gLUC59"
                     /note="from Candida albicans Pga59/orf19.2767 signal
                     peptide and first two amino acid residues of mature domain;
                     PGA59"
     CDS             251..802
                     /label=hGLuc
                     /note="secreted Gaussia luciferase"
     misc_feature    821..1111
                     /gene="gLUC59"
                     /note="from Candida albicans Pga59/orf19.2767 mature domain
                     and GPI-anchor addition domain; PGA59"
     regulatory      1112..1601
                     /note="from CIp10::ACT1p-gLUC59; PGA59"
                     /regulatory_class="terminator"
     primer_bind     complement(1856..1872)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1880..1896)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1904..1934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1949..1970)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2258..2846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3020..3877)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3878..3982)
                     /label=AmpR promoter
     CDS             complement(4090..4890)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4891..5106)
                     /label=URA3 promoter