pY1-gLUC59(GW) vector (V002005)

LOCUS       40924_47438        6819 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pY1-gLUC59(GW), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Bonaldi K, Li Z, Kang SE, Breton G, Pruneda-Paz JL.
  TITLE     Novel surface luciferase reporter for high-throughput quantitative 
            test one-hybrid screens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Pruneda-Paz JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2017) Biological Sciences, University of 
            California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 6819)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6819)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-FEB-2017) Biological Sciences, University of California San 
            Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    30..154
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        179..209
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             263..919
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1264..1566
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(1610..1734)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1759..1897
                     /note="from pLacZi; CYC1"
                     /regulatory_class="promoter"
     misc_feature    1898..1958
                     /gene="gLUC59"
                     /note="from Candida albicans Pga59/orf19.2767 signal
                     peptide and first two amino acid residues of mature domain;
                     PGA59"
     CDS             1964..2515
                     /label=hGLuc
                     /note="secreted Gaussia luciferase"
     misc_feature    2534..2824
                     /gene="gLUC59"
                     /note="from Candida albicans Pga59/orf19.2767 mature domain
                     and GPI-anchor addition domain; PGA59"
     regulatory      2825..3314
                     /note="from CIp10::ACT1p-gLUC59; PGA59"
                     /regulatory_class="terminator"
     primer_bind     complement(3569..3585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3593..3609)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3617..3647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3662..3683)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3971..4559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4733..5590)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5591..5695)
                     /label=AmpR promoter
     CDS             complement(5803..6603)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(6604..6819)
                     /label=URA3 promoter