pXoom-AQP4e vector (V002027)

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LOCUS       V002027                 6182 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002027
VERSION     V002027
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Moe SE, Sorbo JG, Sogaard R, Zeuthen T, Petter Ottersen O, Holen T.
  TITLE     New isoforms of rat Aquaporin-4
  JOURNAL   Genomics 91 (4), 367-377 (2008)
   PUBMED   18255256
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Sorbo JG, Moe SE, Holen TV.
  TITLE     New Isoforms of Rat Aquaporin-4
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6182)
  AUTHORS   Sorbo JG, Moe SE, Holen TV.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2007) CMBN, University of Oslo, Sognsvannsveien 9,
            Oslo, no 0317, Norway
REFERENCE   4  (bases 1 to 6182)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6182)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genomics";
            date: "2008"; volume: "91"; issue: "4"; pages: "367-377"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (12-FEB-2007) CMBN, University of Oslo, Sognsvannsveien 9, Oslo, no
            0317, Norway"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        224..603
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        604..807
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        852..870
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           903..945
                     /label="Xenopus globin 5'-UTR"
                     /note="translational enhancer from the 5'-UTR of the major
                     beta-globin gene of Xenopus laevis"
     CDS             1102..2070
                     /gene="Aqp4"
                     /label="Aquaporin-4"
                     /note="Aquaporin-4 from Rattus norvegicus. Accession#:
                     P47863"
     3'UTR           2153..2278
                     /label="Xenopus globin 3'-UTR"
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     polyA_signal    2424..2648
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2694..3122
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3136..3466
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        3514..3561
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             3580..4284
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             4291..5079
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5256..5389
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5519..6107)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"