pXNS2pat-Strep vector (V002030)

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LOCUS       40924_47238        5316 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant binary vector pXNS2pat-Strep, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Cao FQ, Werner AK, Dahncke K, Romeis T, Liu LH, Witte CP.
  TITLE     Identification and characterization of proteins involved in rice 
            urea and arginine catabolism
  JOURNAL   Plant Physiol. 154 (1), 98-108 (2010)
  PUBMED    20631318
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Cao FQ, Dahncke K, Liu LH, Romeis T, Werner AK, Witte C-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2010) Dahlem Centre of Plant Science, Department 
            of Plant Biochemistry, Freie Universitaet Berlin, 
            Koenigin-Luise-Str. 12-16, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 5316)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5316)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2010"; volume: "154"; issue: "1"; pages: "98-108"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2010) Dahlem Centre of Plant Science, Department of Plant 
            Biochemistry, Freie Universitaet Berlin, Koenigin-Luise-Str. 12-16, 
            Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             39..62
                     /codon_start=1
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
                     /translation="WSHPQFEK"
     primer_bind     complement(94..110)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    393..417
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      529..1238
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1312..1403
                     /label=AmpR promoter
     CDS             1404..2261
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2435..3023
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3335..3359
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3453..3705)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3728..4276)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4319..4498)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4642..5315
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"