pXLJ Con vector (V002032)

LOCUS       V002032                 5073 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002032
VERSION     V002032
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Mokrejs M, Vopalensky V, Kolenaty O, Masek T, Feketova Z, Sekyrova
            P, Skaloudova B, Kriz V, Pospisek M.
  TITLE     IRESite: the database of experimentally verified IRES structures
            (www.iresite.org)
  JOURNAL   Nucleic Acids Res. 34 (DATABASE ISSUE), D125-D130 (2006)
   PUBMED   16381829
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Masek T, Mokrejs M, Vopalensky V, Pospisek M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2008) Genetics and Microbiology, Faculty of
            Science, Charles University in Prague, Vinicna 5, Prague 128 44,
            Czech Republic
REFERENCE   3  (bases 1 to 5073)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5073)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res. 34 (DATABASE ISSUE), D125-D130 (2006)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-JUL-2008) Genetics and Microbiology, Faculty of Science, Charles
            University in Prague, Vinicna 5, Prague 128 44, Czech Republic"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        807..911
                     /label="AmpR promoter"
     CDS             912..1769
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1943..2531
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2783..2801
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2838..4013
                     /gene="ccnb2"
                     /label="G2/mitotic-specific cyclin-B2"
                     /note="G2/mitotic-specific cyclin-B2 from Xenopus laevis.
                     Accession#: P13351"
     CDS             4215..4883
                     /gene="NS"
                     /label="Non-structural protein 1"
                     /note="Non-structural protein 1 from Influenza A virus
                     (strain A/Russia:St.Petersburg/8/2006 H1N1). Accession#:
                     B4URE2"
     promoter        complement(join(5072..5073,1..17))
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"