pXICL vector (V002037)

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LOCUS       40924_47203        4425 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pXICL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ness JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2009) DNA2.0, Inc., 1430 O'Brien Drive, Suite E, 
            Menlo Park, CA 94025, USA
REFERENCE   2  (bases 1 to 4425)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4425)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2009) DNA2.0, Inc., 1430 O'Brien Drive, Suite E, Menlo Park,
            CA 94025, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      13..1490
                     /label=ICL promoter
                     /note="ICL promoter"
                     /regulatory_class="promoter"
     CDS             1493..2200
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      2212..2508
                     /label=ICL terminator
                     /note="ICL terminator"
                     /regulatory_class="terminator"
     promoter        2510..2921
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2929..2976
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2995..3366
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      3435..3682
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3757..4345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"