pXEN vector (V002047)

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LOCUS       40924_47153        3239 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXEN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Czirjak G, Vuity D, Enyedi P.
  TITLE     Phosphorylation-dependent binding of 14-3-3 proteins controls TRESK 
            regulation
  JOURNAL   J. Biol. Chem. 283 (23), 15672-15680 (2008)
  PUBMED    18397886
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Czirjak G, Vuity D, Enyedi P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2007) Department of Physiology, Semmelweis 
            University, Puskin str. 9, Budapest 1088, Hungary
REFERENCE   3  (bases 1 to 3239)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3239)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol. 
            Chem."; date: "2008"; volume: "283"; issue: "23"; pages: 
            "15672-15680"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-NOV-2007) Department of Physiology, Semmelweis University, 
            Puskin str. 9, Budapest 1088, Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           27..60
                     /label=derived from Xenopus globin
                     /note="derived from Xenopus globin"
     misc_feature    61..114
                     /label=multiple cloning site
                     /note="multiple cloning site"
     3'UTR           132..257
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     primer_bind     complement(341..357)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(394..412)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(433..449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(457..473)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(481..511)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(526..547)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(835..1423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1597..2454)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2455..2559)
                     /label=AmpR promoter
     rep_origin      2586..3041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3182..3198
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3208..3226
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"