pXCS-HAStrep vector (V002049)

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LOCUS       40924_47143        5332 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant binary vector pXCS-HAStrep, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Witte CP, Noel LD, Gielbert J, Parker JE, Romeis T.
  TITLE     Rapid one-step protein purification from plant material using the 
            eight-amino acid StrepII epitope
  JOURNAL   Plant Mol. Biol. 55 (1), 135-147 (2004)
  PUBMED    15604670
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Witte C-P., Noel L, Parker JE, Romeis T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2003) Plant-Microbe Interactions, 
            Max-Planck-Institute for Plant Breeding Research, Carl-von-Linne-Weg
            10, Koeln D-50829, Germany
REFERENCE   3  (bases 1 to 5332)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5332)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2004"; volume: "55"; issue: "1"; pages: "135-147"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-NOV-2003) Plant-Microbe Interactions, Max-Planck-Institute for 
            Plant Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             22..48
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             61..84
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     regulatory      90..306
                     /label=pA35S
                     /note="pA35S"
                     /regulatory_class="terminator"
     misc_feature    377..401
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      513..1222
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1296..1387
                     /label=AmpR promoter
     CDS             1388..2245
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2419..3007
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3319..3343
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3437..3689)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3712..4260)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(4303..4482)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4626..5299
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     primer_bind     5302..5318
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"