pXcmkn12 vector (V002050)

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LOCUS       40924_47138        3551 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXcmkn12 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Yasuda S.
  TITLE     Cloning Vector pXcmkn12
  JOURNAL   Published Only in Database (2003)
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Yasuda S, Tamura S, Yamamoto M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2003) Seiichi Yasuda, National Instutute of 
            Genetics, Microbial Genetics; Yata, 1111, Mishima, Shizuoka 
            411-8540, Japan (E-mail:cvector@lab.nig.ac.jp, Tel:81-55-981-6759, 
            Fax:81-55-981-6763)
REFERENCE   3  (bases 1 to 3551)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3551)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-APR-2003) Seiichi Yasuda, National Instutute of Genetics, 
            Microbial Genetics"; volume: " Yata, 1111, Mishima, Shizuoka 
            411-8540, Japan (E-mail:cvector@lab.nig.ac.jp, Tel:81-55-981-6759, 
            Fax"; pages: "81-55-981-6763"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(945..1802)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1803..1907)
                     /label=AmpR promoter
     primer_bind     2381..2397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2401..2430
                     /label=multi cloning site-1
                     /note="multi cloning site-1"
     misc_feature    2435..2449
                     /label=XcmI site-1
                     /note="XcmI site-1"
     CDS             2463..3275
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    3282..3296
                     /label=XcmI site-2
                     /note="XcmI site-2"
     misc_feature    3295..3322
                     /label=multi cloning site-2
                     /note="multi cloning site-2"
     primer_bind     complement(3332..3348)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3356..3372)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3380..3410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3425..3446)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."