pXCEGFPCAH vector (V002051)

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LOCUS       V002051                 6274 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002051
VERSION     V002051
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Nariya H, Miyata S, Kuwahara T, Okabe A.
  TITLE     Development and characterization of a xylose-inducible gene
            expression system for Clostridium perfringens
  JOURNAL   Appl. Environ. Microbiol. 77 (23), 8439-8441 (2011)
   PUBMED   21965407
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Nariya H, Miyata S.
  TITLE     Development of xylose-inducible EGFP fusion Expression Vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6274)
  AUTHORS   Nariya H, Miyata S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2018) Contact:Hirofumi Nariya Graduate School of
            Biosphere Science, Hiroshima University, Laboratory of Food
            Microbiology and Hygiene; 1-4-4, Kagamiyama 1-chome,
            Higashi-Hiroshima, Hiroshima 739-8528, Japan
REFERENCE   4  (bases 1 to 6274)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6274)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2011"; volume: "77"; issue: "23";
            pages: "8439-8441"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (07-APR-2018) Contact:Hirofumi Nariya Graduate School of Biosphere
            Science, Hiroshima University, Laboratory of Food Microbiology and
            Hygiene; 1-4-4, Kagamiyama 1-chome, Higashi-Hiroshima, Hiroshima
            739-8528, Japan"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      266..430
                     /note="ori; derived from Clostridium perfringens plasmid
                     pIP404 (INSD:M32882.1)"
     CDS             complement(493..1713)
                     /codon_start=1
                     /gene="rep"
                     /product="replication enzyme"
                     /label="rep"
                     /note="derived from Clostridium perfringens plasmid pIP404
                     (INSD:M32882.1)"
                     /protein_id="BBD84638.1"
                     /translation="MRNALKLNKKNYIDNIHSRSKGWITRSVIDKKGYSQWHYKYAELK
                     DLDMSDENIYITLNTFYKPCRRLENIKELNTLFIDLDYYKTGKTKDQVLMDLEKNYFNQ
                     SIPIPNYVIDSGRGMYLIWIINAVPSKALPLWKAVQEYLYNQLKYFGADRQALDATRIL
                     RVPGSINSKSKTVVNILDEYEYIYDLREIQNGFLPELKPYERKKGRPSKINYIYRERSL
                     YYGRIQDIIKLCELREYDLKGHRELILFLYRYYLCSFTEDIEKALNDVLELNSMFRQHL
                     SEREVIRATRSAERCYLDKNKQYKYKNETLIELLEITEEEQKYMTIIISKKEYKRRENI
                     RGKKNYQEQLKAKGKATKKEELNVLRKKIKALKEKGFKNKEITLMLEVPIKTLERHITY
                     MKKNGLL"
     gene            complement(493..1713)
                     /gene="rep"
                     /label="rep"
     CDS             complement(2085..3245)
                     /codon_start=1
                     /gene="xylR"
                     /product="transcriptional repressor of the xylose operon"
                     /label="xylR"
                     /note="derived from Clostridium difficile ATCC 9689"
                     /protein_id="BBD84639.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISAI
                     TKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDVGYDYIFSSLSYLDGTI
                     INSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLFTPY
                     YNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSGIIIN
                     NKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIPKIDSD
                     IVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEILQITKD
                     MLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(2085..3245)
                     /gene="xylR"
                     /label="xylR"
     CDS             3531..4244
                     /label="GFP (S65T)"
                     /note="S65T variant of Aequorea victoria green fluorescent
                     protein (Heim et al., 1995)"
     CDS             4245..4271
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             4272..4289
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(4605..5225)
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      5499..6087
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"