pXB300 vector (V002054)

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LOCUS       40924_47118        4697 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pXB300, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Bina XR, Wong EA, Bina TF, Bina JE.
  TITLE     Construction of a tetracycline inducible expression vector and 
            characterization of its use in Vibrio cholerae
  JOURNAL   Plasmid 76C, 87-94 (2014)
  PUBMED    25451701
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Bina JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2014) Microbiology and Molecular Genetics, 
            University of Pittsburgh School of Medicine, 450 Technology Drive, 
            Pittsburgh, PA 15219, USA
REFERENCE   3  (bases 1 to 4697)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4697)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 
            76C, 87-94 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-OCT-2014) Microbiology and Molecular Genetics, University of 
            Pittsburgh School of Medicine, 450 Technology Drive, Pittsburgh, PA 
            15219, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(29..652)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline repressor TetR"
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLKTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPALLRQAIELFEHQGAEPAFLFGLELIICGLEKQLKCESGS"
     promoter        668..723
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     primer_bind     759..775
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1042..1128
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1220..1247
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1266..1357
                     /label=AmpR promoter
     CDS             1358..2215
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2260..2715
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      2826..3414
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3600..3740)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4131..4355)
                     /codon_start=1
                     /gene="sok"
                     /product="plasmid addiction system protein"
                     /label=sok
                     /protein_id="AJA39865.1"
                     /translation="MWTRHRDASWWLMKINLLRGYLLSATQHGNKPPSRHEAESLKRRA
                     HHSPYTCTLTTLTFPENNPLIQTVHGKSV"
     gene            complement(4131..4355)
                     /gene="sok"
                     /label=sok
     CDS             4399..4557
                     /codon_start=1
                     /gene="hok"
                     /product="plasmid addiction system protein"
                     /label=hok
                     /protein_id="AJA39864.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAF
                     MAYESGK"
     gene            4399..4557
                     /gene="hok"
                     /label=hok