pX1918G vector (V002062)

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LOCUS       V002062                 5008 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002062
VERSION     V002062
KEYWORDS    bata-lactamase (bla); catechol-2,3-dioxygenase (xylE); gentamy
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Schweizer HP.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Schweizer HP, Hoang TT.
  TITLE     An improved system for gene replacement and xylE fusion analysis in
            Pseudomonas aeruginosa
  JOURNAL   Gene 158 (1), 15-22 (1995)
   PUBMED   7789804
REFERENCE   3  (bases 1 to 5008)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5008)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (22-NOV-1994) Herbert P. Schweizer, Microbiology "
            SGRef: number: 2; type: "Journal Article"; journalName: "Gene";
            date: "1995"; volume: "158"; issue: "1"; pages: "15-22"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(399..455)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     CDS             665..1585
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"
     promoter        1704..1732
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             1921..2451
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     misc_feature    2718..2774
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2787..2803)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2811..2827)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2835..2865)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2880..2901)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      complement(2984..3011)
                     /label="T2"
                     /note="T2"
                     /regulatory_class="terminator"
     regulatory      complement(3018..3143)
                     /label="T1"
                     /note="T1"
                     /regulatory_class="terminator"
     rep_origin      complement(3189..3777)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3951..4808)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4809..4913)
                     /label="AmpR promoter"