pX1918 with xylE cassette vector (V002063)

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LOCUS       V002063                 4177 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002063
VERSION     V002063
KEYWORDS    beta-lactamase; catechol-2,3-dioxygenase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Schweizer HP.
  TITLE     Two plasmids, X1918 and Z1918, for easy recovery of the xylE and
            lacZ reporter genes
  JOURNAL   Gene 134 (1), 89-91 (1993)
   PUBMED   8244036
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Harayama S, Rekik M, Bairoch A, Neidle EL, Ornston LN.
  TITLE     Potential DNA slippage structures acquired during evolutionary
            divergence of Acinetobacter calcoaceticus chromosomal benABC and
            Pseudomonas putida TOL pWW0 plasmid xylXYZ, genes encoding benzoate
            dioxygenases
  JOURNAL   J. Bacteriol. 173 (23), 7540-7548 (1991)
   PUBMED   1938949
REFERENCE   3  (bases 1 to 4177)
  AUTHORS   Deretic V, Gill JF, Chakrabarty AM.
  TITLE     Pseudomonas aeruginosa infection in cystic fibrosis: nucleotide
            sequence and transcriptional regulation of the algD gene
  JOURNAL   Nucleic Acids Res. 15 (11), 4567-4581 (1987)
   PUBMED   3108855
REFERENCE   4  (bases 1 to 4177)
  AUTHORS   Konyecsni WM, Deretic V.
  TITLE     Broad-host-range plasmid and M13 bacteriophage-derived vectors for
            promoter analysis in Escherichia coli and Pseudomonas aeruginosa
  JOURNAL   Gene 74 (2), 375-386 (1988)
   PUBMED   3149945
REFERENCE   5  (bases 1 to 4177)
  AUTHORS   Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-1993) Herbert P. Schweizer, Microbiology and
            Infectious Diseases, University of Calgary, 3330 Hospital Drive
            N.W., Calgary, Alberta, T2N 4N1, Canada
REFERENCE   6  (bases 1 to 4177)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 4177)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene";
            date: "1993"; volume: "134"; issue: "1"; pages: "89-91"
            SGRef: number: 2; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "1991"; volume: "173"; issue: "23"; pages:
            "7540-7548"
            SGRef: number: 3; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "1987"; volume: "15"; issue: "11"; pages:
            "4567-4581"
            SGRef: number: 4; type: "Journal Article"; journalName: "Gene";
            date: "1988"; volume: "74"; issue: "2"; pages: "375-386"
            SGRef: number: 5; type: "Journal Article"; journalName: "Submitted
            (03-DEC-1993) Herbert P. Schweizer, Microbiology and Infectious
            Diseases, University of Calgary, 3330 Hospital Drive N.W., Calgary,
            Alberta, T2N 4N1, Canada"
            SGRef: number: 6; type: "Journal Article"
            On Dec 16, 1993 this sequence version replaced gi:434649.
FEATURES             Location/Qualifiers
     source          1..4177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(399..455)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     misc_feature    456..544
                     /label="algD DNA from Pseudomonas aeruginosa"
                     /note="algD DNA from Pseudomonas aeruginosa"
     variation       487..488
                     /replace="c"
                     /note="C reported in this position in original algD
                     sequence"
     variation       573
                     /replace="g"
                     /label="C in originally reported sequence"
                     /note="C in originally reported sequence"
     variation       585
                     /replace="g"
                     /label="C in originally reported sequence"
                     /note="C in originally reported sequence"
     regulatory      651..657
                     /gene="xylE"
                     /regulatory_class="ribosome_binding_site"
     CDS             665..1585
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"
     misc_feature    1887..1943
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1956..1972)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1980..1996)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2004..2034)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2049..2070)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2358..2946)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3120..3977)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3978..4082)
                     /label="AmpR promoter"