pWW3804 vector (V002097)

LOCUS       V002097                 5823 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002097
VERSION     V002097
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Tunable expression tools enable single-cell strain distinction in
            the gut microbiome
  JOURNAL   Cell (2017) In press
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5823)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2017)
            In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-MAR-2017) Microbiology "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    17..284
                     /label="split antibiotic resistance marker"
                     /note="split antibiotic resistance marker"
     promoter        complement(285..389)
                     /label="AmpR promoter"
     CDS             complement(811..2733)
                     /gene="tetQ"
                     /label="Tetracycline resistance protein TetQ"
                     /note="Tetracycline resistance protein TetQ from
                     Bacteroides fragilis. Accession#: Q08425"
     rep_origin      complement(3327..3715)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            3886..3995
                     /label="oriT"
                     /note="incP origin of transfer"
     terminator      4150..4177
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      complement(4283..4871)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        4949..5052
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             5053..5709
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     terminator      5783..5817
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"