pWUR772 vector (V002114)

LOCUS       40924_46683       10294 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Regulator-reporter vector pWUR772, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J, 
            Kengen SW.
  TITLE     A growth- and bioluminescence-based bioreporter for the in vivo 
            detection of novel biocatalysts
  JOURNAL   Microb Biotechnol (2017) In press
  PUBMED    28393499
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J, 
            Kengen SW.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2016) Laboratory of Microbiology, Wageningen 
            University and Research, Stippeneng 4, Wageningen 6708 WE, The 
            Netherlands
REFERENCE   3  (bases 1 to 10294)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10294)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-AUG-2016) Laboratory of Microbiology, Wageningen University and 
            Research, Stippeneng 4, Wageningen 6708 WE, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..87
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     regulatory      95..100
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             107..982
                     /label=araC
                     /note="L-arabinose regulatory protein"
     CDS             complement(995..1807)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(1814..1819)
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(1828..2138)
                     /label=PBAD-adapt
                     /note="PBAD-adapt"
                     /regulatory_class="promoter"
     rep_origin      2245..2790
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     regulatory      3060..3370
                     /label=PBAD-adapt
                     /note="PBAD-adapt"
                     /regulatory_class="promoter"
     regulatory      3377..3382
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             3389..4828
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             4844..5764
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             5816..6895
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             6913..7893
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             8075..9184
                     /label=LuxE
                     /note="LuxE"
     terminator      9207..9293
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(9319..9413)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9437..10093)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(10094..10196)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"