pWUR770 vector (V002115)

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LOCUS       V002115                10570 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002115
VERSION     V002115
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J,
            Kengen SW.
  TITLE     A growth- and bioluminescence-based bioreporter for the in vivo
            detection of novel biocatalysts
  JOURNAL   Microb Biotechnol (2017) In press
   PUBMED   28393499
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J,
            Kengen SW.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2016) Laboratory of Microbiology, Wageningen
            University and Research, Stippeneng 4, Wageningen 6708 WE, The
            Netherlands
REFERENCE   3  (bases 1 to 10570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb
            Biotechnol (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-AUG-2016) Laboratory of Microbiology, Wageningen University and
            Research, Stippeneng 4, Wageningen 6708 WE, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..87
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     regulatory      95..100
                     /label="RBS"
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             107..982
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     CDS             complement(995..2083)
                     /gene="leuB"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Escherichia
                     coli (strain K12). Accession#: P30125"
     regulatory      complement(2090..2095)
                     /label="RBS"
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(2104..2414)
                     /label="PBAD-adapt"
                     /note="PBAD-adapt"
                     /regulatory_class="promoter"
     rep_origin      2521..3066
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     regulatory      3336..3646
                     /label="PBAD-adapt"
                     /note="PBAD-adapt"
                     /regulatory_class="promoter"
     regulatory      3653..3658
                     /label="RBS"
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             3665..5104
                     /label="LuxC"
                     /note="LuxC fatty acid reductase"
     CDS             5120..6040
                     /label="LuxD"
                     /note="LuxD acyltransferase"
     CDS             6092..7171
                     /label="LuxA"
                     /note="LuxA luciferase subunit"
     CDS             7189..8169
                     /label="LuxB"
                     /note="LuxB luciferase subunit"
     CDS             8351..9460
                     /label="LuxE"
                     /note="LuxE"
     terminator      9483..9569
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(9595..9689)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(9713..10369)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(10370..10472)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"