pWSTK2 vector (V002121)

LOCUS       V002121                 8491 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002121
VERSION     V002121
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Wu S, Ying G, Wu Q, Capecchi MR.
  TITLE     A protocol for constructing gene targeting vectors: generating
            knockout mice for the cadherin family and beyond
  JOURNAL   Nat Protoc 3 (6), 1056-1076 (2008)
   PUBMED   18546598
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   Wu S, Capecchi MR.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2008) Howard Hughes Medical Institute, University
            of Utah, 15N 2030E, Salt Lake City, UT 84112, USA
REFERENCE   3  (bases 1 to 8491)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8491)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Protoc"; date: "2008"; volume: "3"; issue: "6"; pages: "1056-1076"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-FEB-2008) Howard Hughes Medical Institute, University of Utah,
            15N 2030E, Salt Lake City, UT 84112, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..858
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1032..1620
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        1878..1896
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2361..3488
                     /gene="TK"
                     /label="Thymidine kinase"
                     /note="Thymidine kinase from Human herpesvirus 2 (strain
                     333). Accession#: P04407"
     protein_bind    4253..4377
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4402..4432
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             4486..5142
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             5487..5789
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(5833..5957)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6214..6276
                     /label="Mini-TK promoter"
                     /note="minimal herpes simplex virus (HSV) thymidine kinase
                     promoter"
     5'UTR           6298..6337
                     /label="HSV TK 5'-UTR"
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             6355..7482
                     /label="HSV TK"
                     /note="herpes simplex virus thymidine kinase"
     polyA_signal    7527..7575
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(7897..7913)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8387..8491
                     /label="AmpR promoter"