pWIZdir vector (V002134)

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LOCUS       40924_46558        9017 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWIZdir, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Jenny A, Mlodzik M.
  TITLE     Modified vectors for the two-step directional cloning of inverted 
            repeats for RNA interference in Drosophila
  JOURNAL   BioTechniques 44 (3), 335-339 (2008)
  PUBMED    18361787
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Jenny A, Mlodzik M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2007) Brookdale Department of Molecular, Cell and 
            Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. 
            Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA
REFERENCE   3  (bases 1 to 9017)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9017)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2008"; volume: "44"; issue: "3"; pages: 
            "335-339"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2007) Brookdale Department of Molecular, Cell and 
            Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. 
            Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3057..3151
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3170..3408
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     intron          3468..3541
                     /note="white intron in hairpin"
     intron          3652..3717
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3847..3867
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4139..4273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4293..8421
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(8432..9017)
                     /label=P element 5' end