pWH1274 vector (V002135)

LOCUS       40924_46548        6372 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWH1274, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Wang Y, Chen Y, Zhou Q, Huang S, Ning K, Xu J, Kalin RM, Rolfe S, 
            Huang WE.
  TITLE     A culture-independent approach to unravel uncultured bacteria and 
            functional genes in a complex microbial community
  JOURNAL   PLoS ONE 7 (10), E47530 (2012)
  PUBMED    23082176
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Wang Y, Huang W.
  TITLE     Development of a toolbox for the dissection of naphthalene 
            degradation genes from unculturable microorganisms in complex 
            environment
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6372)
  AUTHORS   Wang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2011) Kroto Research Institute, University of 
            Sheffield, Broad Lane, Sheffield, South Yorkshire S3 7HQ, United 
            Kingdom
REFERENCE   4  (bases 1 to 6372)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6372)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "10"; pages: "E47530"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (20-JUL-2011) Kroto Research Institute, University of Sheffield, 
            Broad Lane, Sheffield, South Yorkshire S3 7HQ, United Kingdom"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             86..1273
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     misc_feature    4219..4359
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4545..5133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5307..6164)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6165..6269)
                     /label=AmpR promoter