pWB101 vector (V002208)

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LOCUS       40924_46198        4892 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pWB101, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Bourn WR, Jansen Y, Stutz H, Warren RM, Williamson AL, van Helden 
            PD.
  TITLE     Creation and characterisation of a high-copy-number version of the 
            pAL5000 mycobacterial replicon
  JOURNAL   Tuberculosis (Edinb) 87 (6), 481-488 (2007)
  PUBMED    17888739
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Bourn WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2007) Medical Virology and Institute of Infectious
            Disease and Molecular Medicine, University of Cape Town, Anzio Road 
            Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 4892)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4892)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Tuberculosis (Edinb)"; date: "2007"; volume: "87"; issue: "6"; 
            pages: "481-488"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JAN-2007) Medical Virology and Institute of Infectious Disease 
            and Molecular Medicine, University of Cape Town, Anzio Road 
            Observatory, Cape Town 7925, South Africa"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2014..3909
                     /note="mycobacterial origin; OriM"
     regulatory      3936..4022
                     /label=BCG hsp60
                     /note="BCG hsp60"
                     /regulatory_class="promoter"
     regulatory      4046..4052
                     /note="strong synthetic Shine Delgarno sequence; RBSA"
                     /regulatory_class="ribosome_binding_site"
     CDS             4062..4775
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
     terminator      4810..4856
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"