pw+SNattB vector (V002217)

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LOCUS       40924_46153        7517 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pw+SNattB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Koch R, Ledermann R, Urwyler O, Heller M, Suter B.
  TITLE     Systematic functional analysis of Bicaudal-D serine phosphorylation 
            and intragenic suppression of a female sterile allele of BicD
  JOURNAL   PLoS ONE 4 (2), E4552 (2009)
  PUBMED    19234596
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Koch R, Ledermann R, Urwyler O, Heller M, Suter B.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2008) Institute for Cell Biology, University of 
            Berne, Baltzerstrasse 4, Bern 3012, Switzerland
REFERENCE   3  (bases 1 to 7517)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7517)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2009"; volume: "4"; issue: "2"; pages: "E4552"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAY-2008) Institute for Cell Biology, University of Berne, 
            Baltzerstrasse 4, Bern 3012, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4809..4842
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4857..5005
                     /label=multiple cloning site
                     /note="multiple cloning site"
     protein_bind    5041..5110
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(5329..5347)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5368..5384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5392..5408)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5416..5446)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5461..5482)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5770..6358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6532..7389)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7390..7494)
                     /label=AmpR promoter