pVSR326 vector (V002223)

Download GeneBank File(.gb)

LOCUS       40924_46123        9152 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Chloroplast transformation vector pVSR326, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Reddy VS, Giovanni F, Leelavathi S.
  TITLE     Chloroplast transformation vector pVSR326
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   Reddy VS, Giovanni F, Leelavathi S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2002) Plant Transformation Group, International 
            Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali 
            Marg, New Delhi, Delhi 110067, India
REFERENCE   3  (bases 1 to 9152)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUL-2002) Plant Transformation Group, International Center for 
            Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New 
            Delhi, Delhi 110067, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..1636
                     /note="chloroplast targeting sequence 1; derived from
                     tobacco"
     regulatory      1661..2132
                     /note="psbA promoter; derived from rice"
                     /regulatory_class="promoter"
     CDS             2133..3941
                     /label=GUS
                     /note="beta-glucuronidase"
     regulatory      4014..4387
                     /note="psbA terminator; derived from rice"
                     /regulatory_class="terminator"
     regulatory      4393..4503
                     /note="rrn promoter; derived from rice"
                     /regulatory_class="promoter"
     CDS             4533..5318
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      5326..5583
                     /note="rbcL terminator; derived from rice"
                     /regulatory_class="terminator"
     misc_feature    5598..6914
                     /note="chloroplast targeting sequence 2; derived from
                     tobacco"
     primer_bind     complement(6931..6947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6955..6971)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6979..7009)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7024..7045)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7333..7921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8095..8952)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8953..9057)
                     /label=AmpR promoter