pVMGCRT85 vector (V002229)

LOCUS       V002229                11368 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002229
VERSION     V002229
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Gao M, Teplitski M.
  TITLE     RIVET-a tool for in vivo analysis of symbiotically relevant gene
            expression in Sinorhizobium meliloti
  JOURNAL   Mol. Plant Microbe Interact. 21 (2), 162-170 (2008)
   PUBMED   18184060
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   Gao M, Teplitski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Soil and Water Science, University of
REFERENCE   3  (bases 1 to 11368)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11368)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant
            Microbe Interact."; date: "2008"; volume: "21"; issue: "2"; pages:
            "162-170"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-OCT-2007) Soil and Water Science, University of Florida-IFAS,
            1376 Mowry Rd., Cancer "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      28..58
                     /label="Escherichia coli trpA"
                     /note="Escherichia coli trpA"
                     /regulatory_class="terminator"
     misc_feature    90..612
                     /note="partial sequence of Sinorhizobium meliloti nodB
                     (Sma0868) and nodC (Sma0866)"
     misc_feature    624..635
                     /label="contains stop codons in all three reading frames"
                     /note="contains stop codons in all three reading frames"
     regulatory      711..717
                     /gene="tnpR"
                     /regulatory_class="ribosome_binding_site"
     CDS             727..1275
                     /gene="tnpR"
                     /label="Transposon gamma-delta resolvase"
                     /note="Transposon gamma-delta resolvase from Escherichia
                     coli (strain K12). Accession#: P03012"
     regulatory      1278..1283
                     /gene="uidA"
                     /regulatory_class="ribosome_binding_site"
     CDS             1310..3118
                     /label="GUS"
                     /note="beta-glucuronidase"
     CDS             3541..4332
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        5345..5373
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             5421..6608
                     /label="TcR"
                     /note="tetracycline efflux protein"
     promoter        7735..7763
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     primer_bind     complement(8287..8303)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      8516..8971
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     oriT            complement(9310..9419)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     promoter        9589..9693
                     /label="AmpR promoter"
     CDS             9694..10551
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      10725..11313
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"