pVLH-1 vector (V002234)

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LOCUS       40924_46028        7823 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVLH-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Deitsch KW, Wellems TE.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-1998) LPD/NIAID, NIH, Bldg 4, Rm B1-34, Bethesda, 
            MD 20892, USA
REFERENCE   2  (bases 1 to 7823)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-JAN-1998) LPD/NIAID, NIH, Bldg 4, Rm B1-34, Bethesda, MD 20892, 
            USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          52..2651
                     /chromosome="12"
                     /strain="Dd2"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:5833"
                     /organism="Plasmodium falciparum"
     regulatory      33..42
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      52..2651
                     /gene="var7b"
                     /label=variant surface antigen regulatory region
                     /note="variant surface antigen regulatory region"
                     /regulatory_class="promoter"
     gene            52..2651
                     /gene="var7b"
                     /label=var7b
     CDS             2662..4308
                     /label=luciferase
                     /note="firefly luciferase"
     primer_bind     complement(4892..4908)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4945..4963)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4981..4997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5005..5021)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5029..5059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5074..5095)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5383..5971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6145..7002)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7003..7107)
                     /label=AmpR promoter
     rep_origin      complement(7188..7643)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7784..7800
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7807..7823
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"