pVLG6 vector (V002235)

LOCUS       V002235                 6607 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002235
VERSION     V002235
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Zeigler DR.
  TITLE     Sequence of pVLG6, a shuttle vector for constructing GFP fusions in
            Bacillus megaterium and other Gram-positive bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Zeigler DR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2011) Bacillus Genetic Stock Center, The Ohio
            State University, 484 W 12th Ave, Columbus, OH 43210, USA
REFERENCE   3  (bases 1 to 6607)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6607)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-APR-2011) Bacillus Genetic Stock Center, The Ohio State
            University, 484 W 12th Ave, Columbus, OH 43210, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..34
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             61..774
                     /label="GFP"
                     /note="green fluorescent protein"
     primer_bind     complement(792..808)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1202..1387
                     /codon_start=1
                     /gene="cop"
                     /product="Cop"
                     /label="cop"
                     /note="copy control regulatory protein for Gram-positive
                     replication"
                     /protein_id="AEP13839.1"
                     /translation="MLGGTVMVVDRKEEKKVAVTLRLTTEENEILNRIKEKYNISKSDA
                     TGILIKKYAKEEYGAF"
     gene            1202..1387
                     /gene="cop"
                     /label="cop"
     CDS             1468..2067
                     /codon_start=1
                     /gene="repF"
                     /product="RepF"
                     /label="repF"
                     /note="initiation protein for Gram-positive replication"
                     /protein_id="AEP13838.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEGRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     gene            1468..2067
                     /gene="repF"
                     /label="repF"
     promoter        complement(2703..2721)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2728..2744)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(2885..3340)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3367..3471
                     /label="AmpR promoter"
     CDS             3472..4329
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4503..5091
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5458..6105)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     protein_bind    6443..6464
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6479..6509
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6517..6533
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6541..6557
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6578..6596
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"