pVK9PtacMuAB vector (V002236)

LOCUS       V002236                10172 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002236
VERSION     V002236
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Gorshkova NV, Gak ER, Tokmakova IL, Mashko SV.
  TITLE     Genetic tools C. glutamicum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   Gorshkova NV, Gak ER, Tokmakova IL, Mashko SV.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2014) Lab3, AGRI, 1st Dorozhny Proezd, 1-1, Moscow
            117545, Russia
REFERENCE   3  (bases 1 to 10172)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10172)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-DEC-2014) Lab3, AGRI, 1st Dorozhny Proezd, 1-1, Moscow 117545,
            Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(463..1857)
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /label="rep"
                     /note="replication protein of pCG1 plasmid from C.
                     glutamicum"
                     /protein_id="AKN19584.1"
                     /translation="MTLADSRDIDTPAWKFSADLFDTHPELALRSRGWTSEDRREFLAH
                     LGRENFQGSKTRDFASAWIKDPDTGETQPKLYRVGSKSLARCQYVALTHAQHAAVLVLD
                     IDVPSHQAGGKIEHVNPEVYAILERWARLEKAPAWIGVNPLSGKCQLIWLIDPVYAAAG
                     MSSPNMRLLAATTEEMTRVFGADQAFSHRLSRWPLHVSDDPTAYRWHAQHNRVDRLADL
                     MEVARMISGTEKPKKRYEQEFSSGRARIEAARKATAEAKALATLEASLPSAAEASGELI
                     DGVRVLWTAPGRAARDETAFRHALTVGYQLKAAGERLKDTKIIDAYERAYTVAQAVGAD
                     GREPDLPPMRDRQTMARRVRGYVAKGQPVVPARQTETQSSRGRKALATMGRRGGKKAAE
                     RWKDPNSEYARAQREKLAKSSQRQARKAKGNRLTIAGWFMTVEGETGSWRQSMKLCLNL
                     ACHVRP"
     gene            complement(463..1857)
                     /gene="rep"
                     /label="rep"
     CDS             complement(2999..3529)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(3718..3746)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     primer_bind     4150..4166
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(4215..5294)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(5295..5372)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        5390..5418
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    5426..5442
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5480..7468
                     /gene="A"
                     /label="DDE-recombinase A"
                     /note="DDE-recombinase A from Escherichia phage Mu.
                     Accession#: P07636"
     CDS             7510..8445
                     /gene="B"
                     /label="ATP-dependent target DNA activator B"
                     /note="ATP-dependent target DNA activator B from
                     Escherichia phage Mu. Accession#: P03763"
     primer_bind     complement(8943..8959)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    8967..8983
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8991..9021)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9036..9057)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9529..10117)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"