pVK-lacIQ-Ptac-MuAB vector (V002237)

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LOCUS       V002237                10173 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002237
VERSION     V002237
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Gorshkova NV, Lobanova JS, Tokmakova IL, Smirnov SV, Akhverdyan VZ,
            Krylov AA, Mashko SV.
  TITLE     Mu-driven transposition of recombinant mini-Mu unit DNA in the
            Corynebacterium glutamicum chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   Gorshkova NV, Lobanova JS, Tokmakova IL, Smirnov SV, Akhverdyan VZ,
            Krylov AA, Mashko SV.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2017) Ajinomoto-Genetika Research Institute, 1st
            Dorozhny pr. 1-1, Moscow 117545, Russian Federation
REFERENCE   3  (bases 1 to 10173)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10173)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-SEP-2017) Ajinomoto-Genetika Research Institute, 1st Dorozhny
            pr. 1-1, Moscow 117545, Russian Federation"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(404..1858)
                     /direction=LEFT
                     /label="ori_pCG1"
                     /note="ori_pCG1"
     CDS             complement(3000..3530)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(3719..3747)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     primer_bind     4150..4166
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(4215..5294)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(5295..5372)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        5390..5418
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    5424..5444
                     /label="O lac binding site"
                     /bound_moiety="O lac"
     protein_bind    5426..5442
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5480..7468
                     /gene="A"
                     /label="DDE-recombinase A"
                     /note="DDE-recombinase A from Escherichia phage Mu.
                     Accession#: P07636"
     CDS             7510..8445
                     /gene="B"
                     /label="ATP-dependent target DNA activator B"
                     /note="ATP-dependent target DNA activator B from
                     Escherichia phage Mu. Accession#: P03763"
     primer_bind     complement(8944..8960)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    8968..8984
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8992..9022)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9037..9058)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9530..10118)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"