pVISIA11 vector (V002238)

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LOCUS       V002238                 7327 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002238
VERSION     V002238
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Campos J, Mongodin EF, Fraser CM, Khan S.
  TITLE     In vivo gastrointestinal evaluation technology: barcoded molecular
            tags to identify genetic determinants of E. coli colonization
            efficiency in mice
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Huang S-H., Wu J, Cappiello C, Patel S, Hand N, Sridharan V,
            Zeichner SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2015) Center for Cancer and Immunology Research,
            Children's National Medical Center, 111 Michigan Ave NW, Washington,
            DC 20010, USA
REFERENCE   3  (bases 1 to 7327)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7327)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-MAR-2015) Center for Cancer and Immunology Research, Children's
            National Medical Center, 111 Michigan Ave NW, Washington, DC 20010,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        133..161
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             209..1396
                     /label="TcR"
                     /note="tetracycline efflux protein"
     promoter        1701..1805
                     /label="AmpR promoter"
     misc_feature    1809..1886
                     /note="CTB signal sequence; Cholera toxin B signal
                     sequence"
     CDS             1923..1949
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    1989..3332
                     /label="beta-barrel of the AIDA-I autotransporter"
                     /note="beta-barrel of the AIDA-I autotransporter"
     CDS             3722..4513
                     /gene="thyA"
                     /label="Thymidylate synthase"
                     /note="Thymidylate synthase from Escherichia coli O139:H28
                     (strain E24377A / ETEC). Accession#: A7ZQT3"
     misc_feature    4705..5364
                     /label="barcode region"
                     /note="barcode region"
     rep_origin      complement(5510..6098)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6292..7149)
                     /label="AmpR"
                     /note="beta-lactamase"
     regulatory      complement(7150..7327)
                     /label="bla promoter"
                     /note="bla promoter"
                     /regulatory_class="promoter"
     promoter        complement(7150..7241)
                     /gene="bla"
                     /label="AmpR promoter"