pVDHS1 vector (V002245)

LOCUS       V002245                 8808 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002245
VERSION     V002245
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Vafadarnejad E, Amoozegar MA, Karimifard A.
  TITLE     Construction of an inducible expression vector for gene knock down
            in Haloarchaeum Halobacterium salinarum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   Vafadarnejad E, Deldar AA, Amoozegar MA, Bazzaz A, Karimifard A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2014) Department of Microbiology, University of
            Tehran, Qods Ave, Tehran 14155-6455, Iran
REFERENCE   3  (bases 1 to 8808)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8808)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-MAY-2014) Department of Microbiology, University of Tehran, Qods
            Ave, Tehran 14155-6455, Iran"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      677..4426
     CDS             4824..6032
                     /gene="hmgA"
                     /label="3-hydroxy-3-methylglutaryl-coenzyme A reductase"
                     /note="3-hydroxy-3-methylglutaryl-coenzyme A reductase from
                     Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879
                     / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2). Accession#:
                     Q59468"
     primer_bind     complement(6376..6392)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(6620..6638)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6659..6675)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6683..6699)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6707..6737)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6752..6773)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7061..7649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7823..8680)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8681..8785)
                     /label="AmpR promoter"