pVCPGUS23010 vector (V002246)

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LOCUS       V002246                14109 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002246
VERSION     V002246
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14109)
  AUTHORS   Singh A, Buttar BS, Gupta A, Khurana JP, Chaudhary VK.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2012) Department of Biochemistry, Department of
            Microbiology and Department of Plant Molecular Biology, University
            of Delhi South Campus, New Delhi, Delhi 110021, India
REFERENCE   2  (bases 1 to 14109)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 14109)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (23-JAN-2012) Department of Biochemistry, Department of Microbiology
            and Department of Plant Molecular Biology, University of Delhi South
            Campus, New Delhi, Delhi 110021, India"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(29..76)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     terminator      complement(143..395)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(415..438)
                     /label="8xHis"
                     /note="8xHis affinity tag"
     CDS             complement(451..480)
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             complement(490..2295)
                     /label="GUS"
                     /note="beta-glucuronidase"
     5'UTR           complement(2328..2364)
                     /label="derived from alfalfa mosaic virus"
                     /note="derived from alfalfa mosaic virus"
     promoter        complement(2369..2387)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2582..2927
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2968..5595
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     terminator      5622..5874
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5896..5920
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7220..7846
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8278..9348
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9417..9611
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9955..10095
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     misc_feature    9985..10245
                     /label="bom site of pBR322 from pCAMBIA-2301"
                     /note="bom site of pBR322 from pCAMBIA-2301"
     rep_origin      complement(10281..10869)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10959..11750)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    12175..12199
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(12277..12451)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12511..13299)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(13368..14045)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"