pVCPGUS(I)23010 vector (V002247)

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LOCUS       V002247                14317 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002247
VERSION     V002247
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14317)
  AUTHORS   Singh A, Buttar BS, Gupta A, Khurana JP, Chaudhary VK.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2012) Department of Biochemistry, Department of
            Microbiology and Department of Plant Molecular Biology, University
            of Delhi South Campus, New Delhi, Delhi 110021, India
REFERENCE   2  (bases 1 to 14317)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 14317)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (23-JAN-2012) Department of Biochemistry, Department of Microbiology
            and Department of Plant Molecular Biology, University of Delhi South
            Campus, New Delhi, Delhi 110021, India"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(29..76)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     terminator      complement(143..395)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(415..438)
                     /label="8xHis"
                     /note="8xHis affinity tag"
     CDS             complement(451..480)
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             complement(490..2313)
                     /label="GUS"
                     /note="beta-glucuronidase"
     intron          complement(2308..2497)
                     /label="cat1 intron"
                     /note="castor bean catalase intron, modified"
     5'UTR           complement(2536..2572)
                     /label="derived from alfalfa mosaic virus"
                     /note="derived from alfalfa mosaic virus"
     promoter        complement(2577..2595)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2790..3135
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3176..5803
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     terminator      5830..6082
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6104..6128
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7428..8054
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8486..9556
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9625..9819
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10163..10303
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10489..11077)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11167..11958)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    12383..12407
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(12485..12659)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12719..13507)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(13576..14253)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"