pVCDcDL3 vector (V002248)

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LOCUS       V002248                 4061 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002248
VERSION     V002248
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Gupta A, Onda M, Pastan I, Adhya S, Chaudhary VK.
  TITLE     High-density functional display of proteins on bacteriophage lambda
  JOURNAL   J. Mol. Biol. 334 (2), 241-254 (2003)
   PUBMED   14607116
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Gupta A, Chaudhary VK.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2002) Biochemistry, University of Delhi South
            Campus, Benito Juarez Road, New Delhi 110021, India
REFERENCE   3  (bases 1 to 4061)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4061)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol.
            Biol."; date: "2003"; volume: "334"; issue: "2"; pages: "241-254"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-DEC-2002) Biochemistry, University of Delhi South Campus, Benito
            Juarez Road, New Delhi 110021, India"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             265..594
                     /gene="D"
                     /label="Capsid decoration protein"
                     /note="Capsid decoration protein from Escherichia phage
                     lambda. Accession#: P03712"
     protein_bind    763..784
                     /label="CAP binding site"
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        799..829
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    837..853
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             873..1046
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
     protein_bind    1346..1379
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      1379..1807
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     protein_bind    complement(1825..1858)
                     /label="loxP511"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     promoter        2156..2260
                     /label="AmpR promoter"
     CDS             2261..3118
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3292..3880
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"