pVCC vector (V002250)

LOCUS       V002250                 5155 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002250
VERSION     V002250
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Vannini A, Agriesti F, Mosca F, Roncarati D, Scarlato V, Danielli A.
  TITLE     A convenient and robust in vivo reporter system to monitor gene
            expression in the human pathogen Helicobacter pylori
  JOURNAL   Appl. Environ. Microbiol. 78 (18), 6524-6533 (2012)
   PUBMED   22773640
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Danielli A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2010) Department of Biology, University of
            Bologna, via Selmi, 3, Bologna, BO 40126, Italy
REFERENCE   3  (bases 1 to 5155)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5155)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "18";
            pages: "6524-6533"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-SEP-2010) Department of Biology, University of Bologna, via
            Selmi, 3, Bologna, BO 40126, Italy"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     1..564
                     /note="flanking region for double homologous recombination
                     in Helicobacter pylori G27 vac::aphA-3/luxCDABE acceptor
                     strain"
     CDS             27..542
                     /codon_start=1
                     /gene="cysS"
                     /product="CysS"
                     /label="cysS"
                     /protein_id="ADZ38982.1"
                     /translation="YLLGVHYRSVLNFNEEDLLVSKKRLDKIYRLKQRVLGTLGGINPN
                     FKKEILECMQDDLNVSKALSVLESMLSSTNEKLDQNPKNKALKGEILANLKFIEELLGI
                     GFKDPSAYFQLGVSESEKQEIENKIEERKRAKEQKNFLKADSIREELLKQKIALMDTPQ
                     GTIWEKFF"
     gene            27..542
                     /gene="cysS"
                     /label="cysS"
                     /note="Helicobacter pylori cysS cistron 5' end"
     CDS             complement(683..1303)
                     /note="Chloramphenicol acetyltransferase from Campylobacter
                     coli. Accession#: P22782"
                     /label="Chloramphenicol acetyltransferase"
     misc_feature    1407..1448
                     /note="MCS; multiple cloning site; unique BamHI, KpnI,
                     SacI, and SnaBI cloning sites"
     gene            1440..2467
                     /gene="luxC"
                     /label="luxC"
                     /note="Photorhabdus luminescens luxC cistron 5' end"
     regulatory      1440..1445
                     /gene="luxC"
                     /regulatory_class="ribosome_binding_site"
     misc_recomb     1451..2467
                     /note="flanking region for double homologous recombination
                     in Helicobacter pylori G27 vac::aphA-3/luxCDABE acceptor
                     strain"
     CDS             1451..2467
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC"
                     /label="luxC"
                     /protein_id="ADZ38983.1"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKES"
     promoter        complement(2476..2494)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2512..2528)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2536..2552)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2560..2590)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2605..2626)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2914..3502)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3676..4533)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4534..4638)
                     /label="AmpR promoter"
     primer_bind     5112..5128
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5135..5153
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"