Basic Vector Information
- Vector Name:
- pVAHS01it
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7530 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Vafadarnejad E, Amoozgar MA, Khansha J, Fallahzade R.
- Promoter:
- lac
pVAHS01it vector Map
pVAHS01it vector Sequence
LOCUS V002257 7530 bp DNA circular SYN 18-DEC-2018 DEFINITION Exported. ACCESSION V002257 VERSION V002257 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 7530) AUTHORS Vafadarnejad E, Amoozgar MA, Khansha J, Fallahzade R. TITLE The rad2 gene of haloarchaeum Halobacterium salinarum is functional in the repair of ultraviolet light induced DNA photoproducts JOURNAL Microbiol. Res. 173, 44-49 (2015) PUBMED 25801970 REFERENCE 2 (bases 1 to 7530) AUTHORS Vafadarnejad E, Amoozegar MA, Danaie Fard M, Khansha J. TITLE Direct Submission JOURNAL Submitted (10-OCT-2014) Department of Microbiology, University of Tehran, Qods Ave., Tehran, Tehran 14155-6455, Iran REFERENCE 3 (bases 1 to 7530) TITLE Direct Submission REFERENCE 4 (bases 1 to 7530) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol. Res. 173, 44-49 (2015)" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (10-OCT-2014) Department of Microbiology, University of Tehran, Qods Ave., Tehran, Tehran 14155-6455, Iran" SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..7530 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(1112..1128) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1136..1152) /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1160..1190) /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind complement(1205..1226) /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1514..2102) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2276..3133) /label="AmpR" /note="beta-lactamase" promoter complement(3134..3238) /label="AmpR promoter" primer_bind 3710..3726 /label="M13 fwd" /note="common sequencing primer, one of multiple similar variants" CDS 4078..5994 /gene="gyrB" /label="DNA gyrase subunit B" /note="DNA gyrase subunit B from Haloferax lucentense (strain DSM 14919 / JCM 9276 / NCIMB 13854 / Aa 2.2). Accession#: P21558" primer_bind complement(6546..6562) /label="SK primer" /note="common sequencing primer, one of multiple similar variants"
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