pVAHS01it vector (V002257)

LOCUS       V002257                 7530 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002257
VERSION     V002257
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Vafadarnejad E, Amoozgar MA, Khansha J, Fallahzade R.
  TITLE     The rad2 gene of haloarchaeum Halobacterium salinarum is functional
            in the repair of ultraviolet light induced DNA photoproducts
  JOURNAL   Microbiol. Res. 173, 44-49 (2015)
   PUBMED   25801970
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Vafadarnejad E, Amoozegar MA, Danaie Fard M, Khansha J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2014) Department of Microbiology, University of
            Tehran, Qods Ave., Tehran, Tehran 14155-6455, Iran
REFERENCE   3  (bases 1 to 7530)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol.
            Res. 173, 44-49 (2015)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-OCT-2014) Department of Microbiology, University of Tehran, Qods
            Ave., Tehran, Tehran 14155-6455, Iran"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1112..1128)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1136..1152)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1160..1190)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1205..1226)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1514..2102)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2276..3133)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3134..3238)
                     /label="AmpR promoter"
     primer_bind     3710..3726
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             4078..5994
                     /gene="gyrB"
                     /label="DNA gyrase subunit B"
                     /note="DNA gyrase subunit B from Haloferax lucentense
                     (strain DSM 14919 / JCM 9276 / NCIMB 13854 / Aa 2.2).
                     Accession#: P21558"
     primer_bind     complement(6546..6562)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"