pUTTnsCm vector (V002265)

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LOCUS       V002265                 8952 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002265
VERSION     V002265
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Li R, Wang G, Shen B, Wang R, Song Y, Li S, Jiang J.
  TITLE     Random transposon vectors pUTTns for the markerless integration of
            exogenous genes into gram-negative eubacteria chromosomes
  JOURNAL   J. Microbiol. Methods 79 (2), 220-226 (2009)
   PUBMED   19778558
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   Li R, Jiang J, Li S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2009) College of Life Science, Nanjing
            Agricultural University, 6# Tongwei Road, Nanjing, Jiangsu 210095,
            China
REFERENCE   3  (bases 1 to 8952)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8952)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Methods"; date: "2009"; volume: "79"; issue: "2"; pages:
            "220-226"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-JUL-2009) College of Life Science, Nanjing Agricultural
            University, 6# Tongwei Road, Nanjing, Jiangsu 210095, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(314..1171)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1172..1276)
                     /label="AmpR promoter"
     CDS             complement(1326..2753)
                     /label="Tn5 transposase"
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
     misc_feature    2871..2889
                     /label="Tn5 IE end"
                     /note="Tn5 IE end"
     CDS             complement(3256..4674)
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     CDS             5333..5980
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    6123..6554
                     /label="similar to sacB"
                     /note="similar to sacB"
     misc_feature    6555..6584
                     /label="MCS"
                     /note="MCS"
     misc_feature    6585..6603
                     /label="Tn5 OE end"
                     /note="Tn5 OE end"
     protein_bind    complement(6615..6631)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(6666..7054)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(7675..8043)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(8076..8185)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"