pUS23 vector (V002273)

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LOCUS       V002273                 4324 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002273
VERSION     V002273
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Coleman NV, Holmes AJ.
  TITLE     The native Pseudomonas stutzeri strain Q chromosomal integron can
            capture and express cassette-associated genes
  JOURNAL   Microbiology (Reading, Engl.) 151 (PT 6), 1853-1864 (2005)
   PUBMED   15941993
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Coleman NV, Holmes AJ.
  TITLE     Capture and expression of an aadB-gfp reporter plasmid by the
            Pseudomonas stutzeri strain Q chromosomal integron
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4324)
  AUTHORS   Coleman NV, Holmes AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2005) School of Molecular and Microbial
            Biosciences, University of Sydney, Building G08, Sydney, NSW 2006,
            Australia
REFERENCE   4  (bases 1 to 4324)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4324)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 151 (PT 6), 1853-1864 (2005)"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (17-JAN-2005) School of Molecular and Microbial Biosciences,
            University of Sydney, Building G08, Sydney, NSW 2006, Australia"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..33
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     terminator      38..124
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      216..243
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     misc_recomb     325..384
                     /label="aadB 59-base element (attC) recombination site"
                     /note="aadB 59-base element (attC) recombination site"
     RBS             402..410
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      403..409
                     /gene="aadB"
                     /regulatory_class="ribosome_binding_site"
     CDS             415..945
                     /gene="aadB"
                     /label="2''-aminoglycoside nucleotidyltransferase"
                     /note="2''-aminoglycoside nucleotidyltransferase from
                     Escherichia coli. Accession#: P0AE04"
     regulatory      955..961
                     /gene="gfpmut3"
                     /regulatory_class="ribosome_binding_site"
     CDS             968..1681
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     primer_bind     complement(1708..1724)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1732..1748)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1756..1786)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1801..1822)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2110..2698)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     regulatory      complement(2722..2759)
                     /label="RNAII"
                     /note="RNAII"
                     /regulatory_class="promoter"
     repeat_region   2722..2759
                     /label="Tn3 terminal inverted repeat"
                     /note="Tn3 terminal inverted repeat"
     CDS             complement(2872..3729)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3730..3834)
                     /label="AmpR promoter"
     primer_bind     4308..4324
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"