pURYL vector (V002274)

LOCUS       40924_45788        5453 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pURYL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Lv H, Gong Z.
  TITLE     Cost-effective and repeatable gene targeting in fission yeast using 
            a cloning-based and modular method
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Lv H, Gong Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 5453)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5453)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAR-2012) School of Life Science, Fudan University, Room 618, 
            Science Building, Handan Road 220, Shanghai 200433, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             292..1002
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
     CDS             1003..1020
                     /label=6xHis
                     /note="6xHis affinity tag"
     regulatory      1030..1522
                     /label=Saccharomyces cerevisiae Leu2 terminator
                     /note="Saccharomyces cerevisiae Leu2 terminator"
                     /regulatory_class="terminator"
     regulatory      1529..2050
                     /label=Schizosaccharomyces pombe Ura4 promoter
                     /note="Schizosaccharomyces pombe Ura4 promoter"
                     /regulatory_class="promoter"
     CDS             2051..2842
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      2846..3275
                     /label=Schizosaccharomyces pombe Ura4 terminator
                     /note="Schizosaccharomyces pombe Ura4 terminator"
                     /regulatory_class="terminator"
     promoter        complement(3342..3360)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3618..4206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4380..5237)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5238..5342)
                     /label=AmpR promoter