pUMRI-12 vector (V002282)

LOCUS       V002282                 6866 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002282
VERSION     V002282
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Xie W, Lv X, Yu H.
  TITLE     pUMRI plasmids for metabolic pathway assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   Xie W, Lv X, Yu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2014) Department of Chemical and Biological
            Engineering, Zhejiang University, Institute of Bioengineering, 34
            TianMuShan Road, Hangzhou, Zhejiang 310028, China
REFERENCE   3  (bases 1 to 6866)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6866)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Artificial Sequence v. Artificial Sequence
            Sequencing Technology :: Artificial Sequence
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-JUL-2014) Department of Chemical and Biological Engineering,
            Zhejiang University, Institute of Bioengineering, 34 TianMuShan
            Road, Hangzhou, Zhejiang 310028, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..55
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(202..771)
                     /gene="PTH1"
                     /label="Peptidyl-tRNA hydrolase"
                     /note="Peptidyl-tRNA hydrolase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P38876"
     misc_feature    complement(796..1499)
                     /label="ERG9 region"
                     /note="ERG9 region"
     regulatory      complement(1497..2613)
                     /label="HXT1 promoter from Saccharomyces cerevisiae"
                     /note="HXT1 promoter from Saccharomyces cerevisiae"
                     /regulatory_class="promoter"
     regulatory      2614..3121
                     /label="GAL7 promoter from Saccharomyces cerevisiae"
                     /note="GAL7 promoter from Saccharomyces cerevisiae"
                     /regulatory_class="promoter"
     promoter        3129..3147
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3165..3194
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     terminator      3224..3413
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(3426..3444)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(3467..3500)
                     /label="Loxp site"
                     /note="Loxp site"
     protein_bind    complement(3467..3500)
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     gene            3546..4902
                     /label="kanMX"
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        4997..5217
                     /label="URA3 promoter"
     CDS             5218..6018
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(6190..6778)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"