pUMLIEP vector (V002285)

LOCUS       40924_45728       14224 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUMLIEP DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14224)
  AUTHORS   Okochi-Takada E, Hattori N, Ito A, Niwa T, Wakabayashi M, Yoshida M,
            Ushijima T.
  TITLE     Establishment of a high-throughput detection system for DNA 
            demethylating agents
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14224)
  AUTHORS   Hattori N, Ushijima T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2016) Contact:Naoko Hattori National Cancer Center
            Research Institute, Division of Epigenomics; 5-1-1 Tsukiji, Chuo-ku,
            Tokyo, Tokyo 104-0045, Japan
REFERENCE   3  (bases 1 to 14224)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14224)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-AUG-2016) Contact:Naoko Hattori National Cancer Center Research 
            Institute, Division of Epigenomics; 5-1-1 Tsukiji, Chuo-ku, Tokyo, 
            Tokyo 104-0045, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    698..6262
                     /gene="UCHL1"
                     /label=UCHL1 5'region
                     /note="UCHL1 5'region"
     gene            698..6262
                     /gene="UCHL1"
                     /label=UCHL1
     misc_feature    6225
                     /gene="UCHL1"
                     /label=UCHL1 TSS
                     /note="UCHL1 TSS"
     CDS             6316..6972
                     /label=MetLuc
                     /note="secreted Metridia luciferase"
     misc_feature    6988..7574
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             7575..8291
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    8417..8538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8562..8578)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8603..9410
                     /gene="UCHL1"
                     /label=contains UCHL1 intron 4
                     /note="contains UCHL1 intron 4"
     gene            8603..9410
                     /gene="UCHL1"
                     /label=UCHL1
     promoter        9420..9749
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             9842..10438
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    10671..10805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    10805..12017
                     /gene="UCHL1"
                     /note="contains UCHL1 intron 4, exon 5, intron 5, exon 6,
                     and intron 6"
     gene            10805..12017
                     /gene="UCHL1"
                     /label=UCHL1
     promoter        complement(12036..12054)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12075..12091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12099..12115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12123..12153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12168..12189)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12477..13065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13239..14096)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(14097..14201)
                     /label=AmpR promoter