pUDE204 vector (V002341)

LOCUS       V002341                11697 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002341
VERSION     V002341
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran JM, Pronk JT, van
            Maris AJ.
  TITLE     Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases
            by alternative pathways for cytosolic acetyl-CoA synthesis
  JOURNAL   Metab. Eng. (2013) In press
   PUBMED   24269999
REFERENCE   2  (bases 1 to 11697)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran J-M.G., Pronk JT,
            van Maris AJA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2013) Biotechnology, Delft University of
            Technology, Julianalaan 67, Delft 2628BC, The Netherlands
REFERENCE   3  (bases 1 to 11697)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11697)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: IDBA v. 0.20
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            (2013) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAY-2013) Biotechnology, Delft University of Technology,
            Julianalaan 67, Delft 2628BC, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(945..1802)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1803..1907)
                     /label="AmpR promoter"
     rep_origin      complement(1934..3276)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     promoter        3538..3753
                     /label="URA3 promoter"
     CDS             3754..4554
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(4688..5143)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5284..5300
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5310..5328
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5838..6357
                     /label="TPI1 promoter"
                     /note="strong constitutive promoter for yeast triose
                     phosphate isomerase"
     CDS             6358..8637
                     /gene="pflB"
                     /label="Formate acetyltransferase 1"
                     /note="Formate acetyltransferase 1 from Escherichia coli
                     (strain K12). Accession#: P09373"
     regulatory      8641..9141
                     /label="GDN2 terminator"
                     /note="GDN2 terminator"
                     /regulatory_class="terminator"
     regulatory      9146..10146
                     /label="FBA1 promoter"
                     /note="FBA1 promoter"
                     /regulatory_class="promoter"
     CDS             10147..10881
                     /gene="pflA"
                     /label="Pyruvate formate-lyase 1-activating enzyme"
                     /note="Pyruvate formate-lyase 1-activating enzyme from
                     Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 /
                     UPEC). Accession#: P0A9N5"
     regulatory      10885..11385
                     /label="PMA1 terminator"
                     /note="PMA1 terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(11386..11402)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(11439..11457)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11478..11494)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    11502..11518
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11526..11556)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11571..11592)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."