pUDE172 vector (V002344)

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LOCUS       V002344                12865 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002344
VERSION     V002344
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Koopman F, Beekwilder J, Crimi B, Houwelingen A, Hall RD, Bosch D,
            van Maris AJ, Pronk JT, Daran JM.
  TITLE     De novo production of the flavonoid naringenin in engineered
            Saccharomyces cerevisiae
  JOURNAL   Microb. Cell Fact. 11 (1), 155 (2012)
   PUBMED   23216753
REFERENCE   2  (bases 1 to 12865)
  AUTHORS   Koopman F, Beekwilder J, Crimi B, van Houwelingen A, Hall RD, Bosch
            D, Maris AJA., Pronk JT, Daran J-M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600,
            The Netherlands
REFERENCE   3  (bases 1 to 12865)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12865)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb.
            Cell Fact."; date: "2012"; volume: "11"; issue: "1"; pages: "155"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..651
                     /label="GAP promoter"
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             657..2831
                     /gene="PAL1"
                     /label="Phenylalanine ammonia-lyase 1"
                     /note="Phenylalanine ammonia-lyase 1 from Arabidopsis
                     thaliana. Accession#: P35510"
     terminator      2835..3082
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     misc_feature    3090..3169
                     /label="recombination site B"
                     /note="recombination site B"
     promoter        3571..4078
                     /label="TPI1 promoter"
                     /note="strong constitutive promoter for yeast triose
                     phosphate isomerase"
     CDS             4078..5592
                     /gene="CYP73A5"
                     /label="Trans-cinnamate 4-monooxygenase"
                     /note="Trans-cinnamate 4-monooxygenase from Arabidopsis
                     thaliana. Accession#: P92994"
     terminator      5734..5921
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    6063..6142
                     /label="recombination site C"
                     /note="recombination site C"
     regulatory      6146..6520
                     /label="PGI1 promoter"
                     /note="PGI1 promoter"
                     /regulatory_class="promoter"
     CDS             6527..8602
                     /gene="ATR1"
                     /label="NADPH--cytochrome P450 reductase 1"
                     /note="NADPH--cytochrome P450 reductase 1 from Arabidopsis
                     thaliana. Accession#: Q9SB48"
     regulatory      8611..8877
                     /operon="PGI1 terminator"
                     /regulatory_class="terminator"
     misc_feature    8917..8996
                     /label="recombination site D"
                     /note="recombination site D"
     CDS             complement(9129..9929)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(9930..10150)
                     /label="URA3 promoter"
     misc_feature    complement(10415..10918)
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        10955..11059
                     /label="AmpR promoter"
     CDS             11060..11917
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      12091..12679
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    12784..12863
                     /label="recombination site A"
                     /note="recombination site A"