pUDE151 vector (V002346)

Basic Vector Information

Vector Name:
pUDE151
Antibiotic Resistance:
Ampicillin
Length:
8101 bp
Type:
Expression vector
Replication origin:
ori
Host:
Yeast
Source/Author:
Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran JM, Pronk JT, van Maris AJ.
Promoter:
GPD

pUDE151 vector Vector Map

pUDE1518101 bp400800120016002000240028003200360040004400480052005600600064006800720076008000oriAmpRAmpR promoter2u oriURA3 promoterURA3f1 oriM13 fwdT7 promoterCYC1 terminatorKS primerattB2Acetaldehyde dehydrogenase (acetylating) EutEattB1SK primerGAP promoterT3 promoterM13 revlac operatorlac promoterCAP binding site

pUDE151 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V002346                 8101 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002346
VERSION     V002346
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran JM, Pronk JT, van
            Maris AJ.
  TITLE     Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases
            by alternative pathways for cytosolic acetyl-CoA synthesis
  JOURNAL   Metab. Eng. (2013) In press
   PUBMED   24269999
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran J-M.G., Pronk JT,
            van Maris AJA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2013) Biotechnology, Delft University of
            Technology, Julianalaan 67, Delft 2628BC, The Netherlands
REFERENCE   3  (bases 1 to 8101)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8101)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            (2013) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAY-2013) Biotechnology, Delft University of Technology,
            Julianalaan 67, Delft 2628BC, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(945..1802)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1803..1907)
                     /label="AmpR promoter"
     rep_origin      complement(1934..3276)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     promoter        3540..3760
                     /label="URA3 promoter"
     CDS             3761..4561
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(4695..5150)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5295..5311
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5321..5339
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(5358..5605)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     primer_bind     5608..5624
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    5657..5681
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5700..7100)
                     /gene="eutE"
                     /label="Acetaldehyde dehydrogenase (acetylating) EutE"
                     /note="Acetaldehyde dehydrogenase (acetylating) EutE from
                     Escherichia coli (strain K12). Accession#: P77445"
     protein_bind    complement(7116..7140)
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7148..7164)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(7170..7814)
                     /label="GAP promoter"
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     promoter        complement(7843..7861)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7882..7898)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7906..7922)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7930..7960)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7975..7996)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."

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