pUDE151 vector (V002346)

LOCUS       V002346                 8101 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002346
VERSION     V002346
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran JM, Pronk JT, van
            Maris AJ.
  TITLE     Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases
            by alternative pathways for cytosolic acetyl-CoA synthesis
  JOURNAL   Metab. Eng. (2013) In press
   PUBMED   24269999
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran J-M.G., Pronk JT,
            van Maris AJA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2013) Biotechnology, Delft University of
            Technology, Julianalaan 67, Delft 2628BC, The Netherlands
REFERENCE   3  (bases 1 to 8101)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8101)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            (2013) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAY-2013) Biotechnology, Delft University of Technology,
            Julianalaan 67, Delft 2628BC, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(945..1802)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1803..1907)
                     /label="AmpR promoter"
     rep_origin      complement(1934..3276)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     promoter        3540..3760
                     /label="URA3 promoter"
     CDS             3761..4561
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(4695..5150)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5295..5311
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5321..5339
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(5358..5605)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     primer_bind     5608..5624
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    5657..5681
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5700..7100)
                     /gene="eutE"
                     /label="Acetaldehyde dehydrogenase (acetylating) EutE"
                     /note="Acetaldehyde dehydrogenase (acetylating) EutE from
                     Escherichia coli (strain K12). Accession#: P77445"
     protein_bind    complement(7116..7140)
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7148..7164)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(7170..7814)
                     /label="GAP promoter"
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     promoter        complement(7843..7861)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7882..7898)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7906..7922)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7930..7960)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7975..7996)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."