pUDE065 vector (V002348)

LOCUS       V002348                12576 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002348
VERSION     V002348
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Koopman F, Beekwilder J, Crimi B, Houwelingen A, Hall RD, Bosch D,
            van Maris AJ, Pronk JT, Daran JM.
  TITLE     De novo production of the flavonoid naringenin in engineered
            Saccharomyces cerevisiae
  JOURNAL   Microb. Cell Fact. 11 (1), 155 (2012)
   PUBMED   23216753
REFERENCE   2  (bases 1 to 12576)
  AUTHORS   Koopman F, Beekwilder J, Crimi B, van Houwelingen A, Hall RD, Bosch
            D, Maris AJA., Pronk JT, Daran J-M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600,
            The Netherlands
REFERENCE   3  (bases 1 to 12576)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12576)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb.
            Cell Fact."; date: "2012"; volume: "11"; issue: "1"; pages: "155"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
            Name: pUDI065.
FEATURES             Location/Qualifiers
     source          1..12576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..1099)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(1112..1516)
                     /label="LEU2 promoter"
     primer_bind     2043..2059
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2069..2087
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2102..2299)
                     /label="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(2361..4043)
                     /gene="4CL3"
                     /label="4-coumarate--CoA ligase 3"
                     /note="4-coumarate--CoA ligase 3 from Arabidopsis thaliana.
                     Accession#: Q9S777"
     promoter        complement(4059..4460)
                     /label="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     primer_bind     4735..4751
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4758..4776
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5200..5708
                     /label="TPI1 promoter"
                     /note="strong constitutive promoter for yeast triose
                     phosphate isomerase"
     CDS             5713..6897
                     /gene="CHS"
                     /label="Chalcone synthase"
                     /note="Chalcone synthase from Arabidopsis thaliana.
                     Accession#: P13114"
     terminator      6907..7094
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        7247..7891
                     /label="GAP promoter"
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             7909..8646
                     /gene="CHI1"
                     /label="Chalcone--flavanone isomerase 1"
                     /note="Chalcone--flavanone isomerase 1 from Arabidopsis
                     thaliana. Accession#: P41088"
     terminator      8662..8909
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(8946..8964)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8985..9001)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    9009..9025
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9033..9063)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9078..9099)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9387..9975)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10149..11006)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11007..11111)
                     /label="AmpR promoter"
     misc_feature    11253..11964
                     /note="partial PYK2 gene; for integration into the PYK2
                     locus"